SeTau-647-NHS

Basic Information

SeTau-647-NHS
K9-4149
1846.36g / Mol
Squaraine
Extremely bright, water-soluble, amine-reactive label containing one NHS-ester group. The ideal label for proteins and other amino-modified biomolecules including oligonucleotides. Brightest label currently available for the 647 nm laser!

Overview

648 nm
694 nm
phosphate buffer PH 7.40
200000 M-1cm-1

Absorption

648 nm
200000 M-1cm-1
-

Emission

694 nm
no
-
-

More parameters

phosphate buffer PH 7.40
20.00° C

spectra uploaded by Ewald Terpetschnig

Provide more Spectra

Quantum Yields

61%
Reference Standard
Alexa 647
phosphate buffer
7.40
20.00° C

Lifetimes

3.20 ns
Phase-modulation Fluorimetry
ISS
phosphate buffer
7.40
20.00° C
https://www.setabiomedicals.com/files/Pdf/K9-4149.pdf

Availabilities

Commercial
SETA BioMedicals
https://www.setabiomedicals.com/squaraine-rotaxanes.html
orders@setabiomedicals.com

Applications

Microarray
https://www.setabiomedicals.com/squaraine-rotaxanes.html
In-vivo imaging
Susan M. Clardy et al. "Two-photon imaging of pancreatic beta cells in real time in vivo". Technology 04, 130 (2016).
Two-photon Imaging
Podgorski K. et al. Ultra-bright and -stable red and NIR squaraine fluorphores for In-vivo 2-photon imaging. PLoS ONE 7(12): e51980 (2012).
labelled antibody
http://www.pnas.org/content/113/12/3323.abstract
Nature paper on kinetics of Megalin endocytosis, recycling and degradation using SeTau 647
A. E. Perez Bay et al. „The fast-recycling receptor Megalin defines the apical recycling pathway of epithelial cells“. Nat. Commun. 7:1155 (2016).
Reactive label
https://www.setabiomedicals.com/k9-4149.html
Protein label
https://www.setabiomedicals.com/k9-4149.html
Synthesis of labeled oligonucleotides
https://www.setabiomedicals.com/squaraine-rotaxanes.html
Nucleic acid label
https://www.setabiomedicals.com/squaraine-rotaxanes.html
2-photon-imaging
Susan M. Clardy et al. "Two-photon imaging of pancreatic beta cells in real time in vivo". Technology 04, 130 (2016).
2-photon-microscopy
Susan M. Clardy et al. "Two-photon imaging of pancreatic beta cells in real time in vivo". Technology 04, 130 (2016).
Microarray
https://www.setabiomedicals.com/squaraine-rotaxanes.html
2-photon-Imaging Dye
Susan M. Clardy et al. "Two-photon imaging of pancreatic beta cells in real time in vivo". Technology 04, 130 (2016)