Rhodamine pH-Probe 585-7.5

Basic Information

Common name(s): Rhodamine pH-Probe 585-7.5

IUPAC name: N,N’-di(3-azapentane-1,5-diyl)-2’,4’-dicarboxyrhodamine

CAS number:

Chemical Formula: C29H29N4O5

Molecular Weight: 513.60g / Mol

Substance Class: Xanthen

Description: Can be grafted to amino groups via DCC/NHS; pH Indicator - complete fluorescence quenching at basic pH, pKa 7.4, absorption is red-shifted by 20nm. Forms lactone in basic and apolar media.

References

D.Aigner et al., Talanta (2012), 99, 194-201., DOI: 10.1016/j.talanta.2012.05.039

Overview

Absorption λ_max

531 nm

Emission λ_max

561 nm

Solvent

Water

Molar Abs. Coefficient

85000 M^-1cm^-1

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Absorption

λ_max

531 nm

Molar Abs. Coefficient

85000 M^-1cm^-1

Instrument

Varian Cary 50 UV-VIS

Emission

λ_max

561 nm

Spectral correction

yes

Excitation wavelength

Instrument

Hitachi F7000

More parameters

Solvent

Water

Temperature

References

D.Aigner et al., Talanta (2012), 99, 194-201., DOI: 10.1016/j.talanta.2012.05.039

spectra uploaded by Daniel Aigner

Quantum Yields

Quantum Yield: 67%

Method: Reference Standard

Standard: Rhodamine 101

Instrument: Hitachi F-7000

Solvent: Water

pH Value: 5.00

Temperature:

Reference:

DOI:

new quantum yield

Lifetimes

No lifetimes information yet.

new lifetime

Availabilities

Type: Academic sharing

Name: Sergey M Borisov

Web address:

E-mail: sergey.borisov@tugraz.at

new availability

Applications

Application: pH-Indicator

Reference:

DOI:

Application: fluorescent labeling

Reference:

DOI:

new application